Journal: Blood

Article Title: Characterization of DLBCL with a PMBL gene expression signature.

doi: 10.1182/blood.2020007683

Figure Lengend Snippet: Figure 5. IL4R mutations in nm-PMBLsig1 tumors induce JAK-STAT activation. (A) Mutational pattern of IL4R (UniProtKB-P24394) in nm-PMBLsig1 tumors (top) and other DLBCL tumors (bottom) from the BCC cohort specified according to receptor domains (extracellular, transmembrane, or cytoplasmic). Colors and shapes of lollipops represent the DLBCL90 NanoString assay tiered classification of samples (see Figure 1) and type of mutation (circle, missense or in-frame indel; diamond, truncating), respectively. The plot was created using R package TrackViewer (version 1.24.0). (B-G) Ectopic expression of WT IL4R and mutants in HEK Blue IL-4/IL-13 (Invivogen) (B-C) and DEV cells (D-G). (B) Quantification of pSTAT6-dependent expression of secreted embryonic alkaline phosphatase (SEAP) in supernatant. (C-D) Immunoblot for pSTAT6, STAT6, IL4R, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (E-F) CCL17 and CD23 messenger RNA expression measured by quantitative polymerase chain reaction. (G) IL4R surface expression measured by flow cytometry. Data are means 6 standard deviation of 5, 4, 7, and 7 experiments in panels A, E, F, and G, respectively; significance was evaluated using a 1-sample Student t test. *P , .05, **P , .01, ****P , .0001.

Article Snippet: Western blotting and flow cytometry were performed as previously described.31 Briefly, membranes were probed with the following primary antibodies at 1:1000 dilution ratio unless stated otherwise: phosphorylated STAT6 (pSTAT6; 9364; Cell Signaling Technology), STAT6 (ab32108; Abcam), and IL4 receptor (IL4R; sc-28361; 1:500; Santa Cruz Biotechnology).

Techniques: Activation Assay, Mutagenesis, Expressing, Western Blot, RNA Expression, Real-time Polymerase Chain Reaction, Cytometry, Standard Deviation

Journal: Cell reports

Article Title: Mass Cytometry Reveals Global Immune Remodeling with Multi-lineage Hypersensitivity to Type I Interferon in Down Syndrome

doi: 10.1016/j.celrep.2019.10.038

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit monoclonal anti-human phospho-STAT6 (Tyr641) (clone 18) , Fluidigm , Cat#3168012A; RRID:AB_2811103.

Techniques: Recombinant, Mass Cytometry, Software